Hi, we routinely use Dako's anti-bcl-2-FITC and Intra-Stain permeabilizing solution according to manufacturer's recommendations -it works very well and if properly standardised can be applied for quantitation. Anna PS. Best wishes to everybody on the Cytometry Mailing List for the Y2K!!!!! At 22:29 1999-12-22 +0100, you wrote: > Dear flowers, previously designed experimental conditions, by flow >cytometry. What would be the most appropriate method for intracellular >bcl-2 detection with FACS? Is it possible to follow BD permeabilization >procedure designed for intracellular cytokine staining (without >stimulation, of course)? Could anyone suggest something special, >experienced with Sigma's anti-bcl-2 mAb and what would be the expected >bcl-2 staining values for above mentioned nontreated cell suspensions? >Thanks in advance Viktor > Dr Jovic Viktor >Dpt. for Pathology and Cytology >Experimental laboratory >INSTITUTE OF ONCOLOGY AND RADIOLOGY >If everything seems to be going so well, you have obviously >overlooked something! > Anna Porwit Heamatopathology Lab. Department of Pathology Karolinska Hospital, Stockholm anpo@mb.ks.se tel.:+46-851774518 fax.:+46-851775843
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