Essentially any will do. PBS-buffered 4% formaldehyde gives more stable slides and permeabilizes cells. Saverio Alberti Head, Lab. of Experimental Oncology Department of Cell Biology and Oncology Consorzio Mario Negri Sud 66030 Santa Maria Imbaro (Chieti), Italy Phone: (39-0872) 570.293 FAX: (39-0872) 570.412 E-mail: alberti@cmns.mnegri.it On Wed, 22 Dec 1999, Grace Jones wrote: > > What is the best way to fix cells expressing a GFP marker, so that one can > counter stain with PI or do some fluorescent immunocytochemistry, but yet > the fix does not kill the GFP marker? > > Grace Jones > >
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