Grace, You can use a vector that has a signal that anchors the GFP into the membrane. Vincent Sahi Columbia University Flow Cytometry -----Original Message----- From: Grace Jones [mailto:gjones@pop.uky.edu] Sent: Wednesday, December 22, 1999 11:46 PM To: cyto-inbox Subject: What is the best way to fix cells expressing a GFP marker, so that one can counter stain with PI or do some fluorescent immunocytochemistry, but yet the fix does not kill the GFP marker? Grace Jones
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