Jim - greetings. No disagreements here. -SNIP- At 02:41 PM 12/21/99 -0600, you wrote: >The width of the beam is not as big an issue as you have made it for >pulse-width time-of-flight discrimination of doublets. Sure-but it can help. >If the laser beam width is subtracted from the pulse width time-of-flight signal in real time using a good biased amplifier, resolution is not a problem. The limits of detection depend only on how good your hydrodynamic focusing is to deliver all of the cells or objects to the same position in the beam at a steady velocity. For successful doublet correction the only limit is that the heterogeneity of nuclear sizes (if you do fluorescence pulse width time-of-flight) or cell sizes (if you do light scatter pulse width time-of-flight). The implementation in commercial systems is the problem. -/SNIP- I absolutely agree. My point, made less than pellucidly, was that DNA flow cytometry is not just immunophenotyping in the red (or blue, or whatever) channel. Thanks for your useful input. Best wishes, Nick Nicholas Terry, M.A., Ph.D., Experimental Radiation Oncology - 066, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, Texas 77030. 'Phone: 713-792-3424, 'Fax: 713-794-5369. http://drad52.mdacc.tmc.edu. ntflow@odin.mdacc.tmc.edu.
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