>Hi, > >What do people think is the most reliable way to check for murine cell >contamination in a human cell line by flow? > Ron, Unfortunately, I've had this very problem in the past - to distinguish mouse from human cells, I've had good luck using species-specific antibodies against MHC class I - W632 (I think) for human and M1/42 for mouse are good pan-class I species-specific Ab. AW Andrew D. Wells, Ph.D. University of Pennsylvania Department of Medicine 904 Stellar-Chance Laboratories 422 Curie Boulevard Philadelphia, PA 19104 (215) 898-1951 (215) 573-2880 (FAX) adwells@mail.med.upenn.edu
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