Species-specific > antibodies against MHC class I is a good idea - just be careful in > interpreting lack of staining with the anti-mouse, as some cell types may > express class I antigens below the limit of detection by flow. > Good luck, Rachel ======================================================= Rachel M. Gerstein, Ph.D. Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) > ---------- > From: Andrew D. Wells, Ph.D. > Sent: Friday, December 10, 1999 1:13 PM > To: Cytometry Mailing List > Subject: Re: Human vs murine > > > >Hi, > > > >What do people think is the most reliable way to check for murine cell > >contamination in a human cell line by flow? > > > > > Ron, > > Unfortunately, I've had this very problem in the past - to distinguish > mouse from human cells, I've had good luck using species-specific > antibodies against MHC class I - W632 (I think) for human and M1/42 for > mouse are good pan-class I species-specific Ab. > > AW > > > > Andrew D. Wells, Ph.D. > University of Pennsylvania > Department of Medicine > 904 Stellar-Chance Laboratories > 422 Curie Boulevard > Philadelphia, PA 19104 > (215) 898-1951 > (215) 573-2880 (FAX) > adwells@mail.med.upenn.edu > > >
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