Hello all: I'm trying to measure apoptosis induced by taxol (paclitaxel) and some other chemicals in MCF-7 cells by 2-color TUNEL, in order to correlate apoptosis and cell cycle. I used PI to stain the DNA. I have tried several doses and treatment time of taxol, but didn't get any positive TUNEL labelling. My other chemicals all showed positive TUNEL labelling, and taxol did show G2/M arrest and later sub-G1 peak as reported by others and as indicated by FL2. But NO TUNEL positive population! I noticed some discussion of "taxol and TUNEL incompatibility" in the mailing list a couple of years ago. I wonder if any one had worked with TUNEL and taxol and would help me out? Taxol is supposed to be my positive control. Others prepared slides and reported TUNEL positive results with the same doses and treatment time as I did. Is it my method, or is the TUNEL doesn't work well with taxol? Any suggestions on 2-color TUNEL is appreciated! thanks Nan Lin
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:54:20 EST