Ron, If you have any clue as to what murine cells are potentially contaminating the human line, try an anti-H2K or anti-H2D antibody since most cells express class I MHC. Do you have any idea about the contamination? Randy T. Fischer TherImmune Research Corporation 9700 Great Seneca Hwy Rockville, MD 20850 (240) 453-6256 RFischer@therimmune.com > ---------- > From: ronald hill > Sent: Thursday, December 9, 1999 10:42 AM > To: Cytometry Mailing List > Subject: Human vs murine > > > Hi, > > What do people think is the most reliable way to check for murine cell > contamination in a human cell line by flow? > > Thanks. > > -Ron Hill > > > Ronald J. Hill, Ph.D. > Group Leader, Immunology Targets > Sugen, Inc. > 230 East Grand Avenue > South San Francisco, CA 94080-4811 > > Tel: (650) 553-8797 > Fax: (650) 553-8313 > E-mail: ron-hill@sugen.com > > >
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