Dear Eugene, I recommend to our users that always wait for a few seconds before acquiring samples allowing stabilization pressure. You can see it looking at sample rate. With the new faster Mac, this problem is more noticeable than older computers. Best regards Marco A. Fernández Head Cytometry Unit University Hospital Germans Trias i Pujol 08916 Badalona (Barcelona) Spain marcoa@ns.hugtip.scs.es http://personal.redestb.es/marcoafdez ---------- >De: Pizzo@nso1.uchc.edu (Pizzo,Eugene) >Para: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Asunto: Calibur data acquisition >Fecha: miér., 8 dici 1999 15:31 > > > > Colleagues, > > Recently we purchased a new FACSCalibur which runs Cellquest on a 400MHz G3. > I noticed that during initial acquisition of a sample there is a 'spurt' of > activity that > produces an image of low fsc vs. ssc on such a plot which within several > seconds > equilibrates to a normal appearance of midscale when acquiring normal mouse > PBL's. > BD tells us and I agree that this is simply a result of the machine > equilibrating pressure > and that most wait a few seconds before acquiring after placing the sample > on the > intake port. In fact this also relates to the question that has appeared > elsewhere on this list concerning > carryover from sample to sample. It is clear that this fact was not > apparent on older machines > because the equilibration had resolved itself before the slower processors > could pick it up. > Thus my question is, was it necessary to wait a few seconds on the older > machines anyway > because the finalization of the 'data-values' was commited to by the > cytometer or wasn't it > necessary because only now with the more rapid CPU does it become truly > necessary to > wait a few seconds for equilibration prior to acquisition? > > Gene/UCONN Health > >
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