Is it made up against a peptide or the whole CD64 molecule? The latter has a better chance of cross reacting. Is it affinity purified? That makes a big difference. I have had excellent experience with polyclonal secondary antibodies. My experience with primary ones is more limited, but many work well are at least have low noise. I do not have much experience with goat primary polyclonals. you may wish to check with Barb and see what anti-goat Ig conjugates we have. You are welcome to try one, if we have it. I would not label it directly until you know if it works in indirect staining. Have you looked through Linscott's directory? They have a list of all commercially available Abs. There is one in the NIH library and I would guess someone at Twinbrook has it as well. After you check Linscott's, I would ask these email discussion groups if anyone knows: NIH immunology interest group: Immuni-L@list.nih.gov Perdue university flow cytometry list: cytometry@flowcyt.cyto.purdue.edu > ---------- > From: Michael Woolhiser > Sent: Wednesday, December 8, 1999 11:03 AM > To: Calman Prussin > Subject: anti-CD64 > > Hi. Thanks again for your time yesterday. The goat polyclonal I found on > the Internet site is indeed directed against human CD64 but the tech. > people suspect it is cross reactive with murine. As far as I can tell this > is based solely on sequence homology since they have not conducted flow or > blot analyses. It is also unlabeled. So...what is your experience with > polyclonal antibodies in FACS analyses? Also, would it be better to order > a secondary antibody or label the polyclonal ourselves? > > Mike Woolhiser >
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