Chris lena wrote:
> Hello gang,
> My question deals with our FacStar. I am getting reports of counts that are
> about 1/2 of the cell number the instrument says were recovered. I am
> wondering if anyone has seen this or what to do to fix this problem. Cell
> count is very important in many of our studies.
> Thanks in advance and Happy Holidays,
>
> chris
>
> Chris Lena
> Laboratory Technician
> La Jolla Institute For Allergy and Immunology
> phone:(858)678-4536
> fax:(858)678-4595
> cali@liai.org
> http://www.liai.org
>
> _ _
> (_)-(_)
> \"/
> =V=
>
>
Hi Chris,
Give me a call at 784-8251 if you like, and you can make arrangemenets to
drop by see how we (often) monitor recovery/yield when these issues come up.
In
short, without more specific info on your setup, it could be timing, stream
stability,
sticky tubes, poor centrifugation, etc.... You need to arm youself with
methodology
to identify where the cells go, and where they don't.
Regards,
Joe
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:54:17 EST