vacuum on a Vantage

From: ray hester (rhester@jaguar1.usouthal.edu)
Date: Mon Nov 29 1999 - 10:35:36 EST


Hi,

We have a FACSVantage and have recently been having problems with drainage
of the center stream, i.e., after thirty minutes or an hour the drainage has
stopped, and we then have sheath fluid overflowing the center stream
collector and fluid filling the basin below.  Since we don't have house
vacuum, we use a small Gast pump as the vacuum source and for awhile that
worked well until we noticed water was getting into the pump.  We then
installed a trap in between the Vantage and the pump and for awhile that
helped but the problem eventually re-surfaced.  Next we filled the trap (a
large, Erlenmeyer flask) with Drierite and that seemed to help for awhile
but now nothing seems to work.

Does anyone have a similar situation - Vantage and no house vacuum?  If so,
please let us know what your setup is.  Perhaps the pump has been damaged
beyond repair. Although it feels as if there is sufficent vacuum, I haven't
actually checked to see how much vacuum there is and this is one of those
small pumps without the gauges.

Thanks for any ideas.

Ray



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