There is another alternative to PerCP for 4-color analysis on the FACSCalibur. PE-Cy7 is available from Caltag Laboratories and was formulated specifically to get around this problem. The diode laser on the FACSCalibur does excite Cy5, causing compensation problems when using it with APC. Cy7, however, is not excited by the red diode. There is very little if any compensation between FL3 and FL4 with this conjugate, and about 30% between FL2 and FL3, For a list of antibodies conjugated to this fluorochrome, check our Website at www.caltag.com. Karen R. Tamul, MS, MT(ASCP)SI Mid-Atlantic Territory Manager Caltag Laboratories Inc.
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