Hi Gayle,
What kind of machine do you have? If it's a B-D one, there probably isn't
a direct FL3-%FL1 compensation circuit. You'll need to compensate through
the FL2-%FL1 and FL3-%FL2, ie compensate so that the signal is removed from
FL2 first, then compensate FL3 against FL2 until it disappears from FL3 as
well. If you're not using an FL2 reagent you might be able to skip the
first step.
Ray
At 10:41 am -0800 24/11/99, Gayle Hatleberg wrote:
>I am relatively new to the Flow field and I have been working with a
>Fluorescein like dye that seems to be contaminating our Red signal. The
>histogram of FL3 630/32 is very broad starting at 10^o and ending at
>10^3? Does anyone have any ides how to compensate out the signal. I
>have tried and the signal is not removed when compensating. Is there a
>good way to remove green signal that is contaminating the red channel?
>Thanks for your help
>Gayle Hatleberg
>Molecular Probes.Inc.
>Gayle@probes.com
Ray Hicks
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