Compensation for Green contaminating RED channel

From: Gayle Hatleberg (GayleHatleberg@probes.com)
Date: Wed Nov 24 1999 - 13:41:19 EST


I am relatively new to the Flow field and I have been working with a
Fluorescein like dye that seems to be contaminating our Red signal.  The
histogram of FL3 630/32 is very broad starting at 10^o and ending at
10^3?  Does anyone have any ides how to compensate out the signal.  I
have tried and the signal is not removed when compensating.  Is there a
good way to remove green signal that is contaminating the red channel?
Thanks for your help
Gayle Hatleberg
Molecular Probes.Inc.
Gayle@probes.com



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