Dear Kevin: Coulter-Immunotech and many other companies are very good about sending data sheets,etc. if you call them up and ask them for a look. That is exactly how I received my first information. The F and P reagents that I described are based on the data sheet and my other experience with saponin-based lysing solutions from my old lab in Dallas. I don't think you will need to buy the kit to get the protocol. Give them a call and see what happens. I would be surprised if you couldn't, but .... maybe you could include your FAX number in your e-mail address and we could exchange some information and references. Dr. Susan J. Zunino Lehrstuhl fur Genetik University of Erlangen Staudtstr. 5 91058 Erlangen Germany Tele. 49 9131 8528784 FAX 49 9131 8528526 e-mail: szunino@biologie.uni-erlangen.de
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