Dear Dr Preffer, Although it may not be the exact answer for cytotoxicity, I remember a paper which describes a multi-color staining method to study heterotypic cell conjugates formation : A multiparameter flow cytometric method to study surface molecules involved in interactions between subpopulations of cells. Aubry JP et al.(1993), J. Immunol. Methods, 159:161-71. May-be it contains some helpful hints ? Hope that helps Philippe Poncelet, PhD Director, Research and Technology BioCytex 140, Chemin de l'Armée d'Afrique 13010 Marseille France Tel: +33 (0) 4 96 12 20 40 Fax: +33 (0) 4 91 47 24 71 www.biocytex.com
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