We tried to find some of those dishes for experiments on the ACAS. We never found a source, but we simply made them. It took a bit of work since the dishes tend to crack if not suported when drilled. we constructed a stand and user a special bit (Unibit Step 3) which is specially designed to bore large holes in thin material. We then used super glue to hold the cover slip in place. There was information from the now defunct Meridian Instruments that the silicone cement had toxic prodducts in it. We found our cells grew well using either type of glue and the silicone gave better sealing. After drilling and gluing, we left the dishes air dry overnight then sterilized them using Ethylene Oxide sterilizers or by irradiating the dishes with CO60. All seemed to work well but there is significant time involved in the process. Charles A. Kuszynski, Ph.D. Assistant Professor/Director University of Nebraska Medical Center 986495 Nebraska Medical Center Cell Analysis Facility, WH 3024 Omaha, NE 68198-6495 402.559.6267 voice 402.559.4077 fax ckuszyns@unmc.edu
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