RE: Cyto Chex Reagent

From: Gaffin, Stephen L USARIEM (Stephen.Gaffin@na.amedd.army.mil)
Date: Fri Sep 24 1999 - 07:39:36 EST


I tried it out in my lab in Massachusetts to permit fedexing samples from
the South.  A single sample of human blood (mine) was treated with CytoChex
on day zero and then analyzed daily for the next 4 days.  The results of
CD4+ and CD8+ were significantly different on day 1 and subsequent days.  As
a result, despite our great hopes according to  claims made, we did not
adopt its use.
Gaffin
************************************************************
************************************************************ 
** Stephen L. Gaffin, Ph.D.
** 
** Research Physiologist
** 
** U.S. Army Research Institute for Environmental Medicine  ** 
** Natick MA  01760-5007
** 
** Tel. 508-233-4867       Fax 508-233-4869                            ** 
** email  stephen.gaffin@na.amedd.army.mil          **
***********************************************************
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		-----Original Message-----
		From:	Abby Kelliher [mailto:allena@helix.mgh.harvard.edu]
		Sent:	Tuesday, September 21, 1999 3:48 PM
		To:	Cytometry Mailing List
		Subject:	Cyto Chex Reagent


		Hello -
		I was wondering if any of you have had experience using the
"Cyto Chex
		Reagent" manufactured by Streck laboratories for preserving
white blood
		cells for flow analysis up to one week later?  I have
received a sample and
		my initial experiment (on a pre B-ALL bone marrow) showed
excellent
		results.  I am going to try it on a variety of specimens,
but I wonder what
		experiences others have had with this product.

		thanks!

		Abby Kelliher
		Clinical Flow Cytometry Lab
		Mass. General Hospital
		Boston, MA 02114
		



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