Dear Flow-ers, I am writing to see if any of you have any suggestions for how to simultaneously stain peripheral blood or bone marrow for a cell surface marker and for nuclear b-galactosidase encoded by a retroviral vector. Most of the b-gal antibodies I have tried yield a large amount of background (Monosan, Sigma, Gibco, 5'-3'), and the fixation and permeabilization required for antibody access to the nucleus (saponin, triton, tween) all seem to disrupt binding of the antibody to the surface marker. Any suggestions would be greatly appreciated. Graça -- Graça Almeida-Porada, M.D., Ph.D Assistant Professor University of Nevada School of Medicine Department of Medicine (151B) VA Medical Center 1000 Locust St Reno NV 89520 Phone 775-328 1232 Fax 775-328-1745
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