Dax Arguello wrote: > I have noticed a problem with carryover on BD instruments, even when running > manually. We do mostly stem cell work here, and it's always necessary to run > bleach and water before the CD34 tubes, since we experience carryover of the > CD33 PE from the previous tube if we don't. It's not inconvenient, but > presents a problem with automation using the loader, which may be why we > haven't gotten one. Before I moved to HCI, I was using a Calibur with a > loader, and noticed similar carryover. I resorted to running manually, and > leaving the sample arm open for several seconds between tubes. Pretty easy > solution, but it does add time to the run. > Can't you do the same thing with Loader Manager by adding mixing time between each tube? John -- ******************************************************************************* John D. Altman, Ph.D. Emory University Vaccine Center at Yerkes 954 Gatewood Road Atlanta, GA 30329 Office: (404) 727-5981 FAX: (404) 727-9005 Lab: (404) 727-8914 email: altman@microbio.emory.edu ******************************************************************************* Tetramer Core Facility: http://www.niaid.nih.gov/reposit/tetramer/index.html *******************************************************************************
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