If the system is to maintained sterile then you need not change filters and chance contaminating the system. The large Pall filter can be used with >20 liters of sheath without having to be changed. I use a (PALL)Gelman Versacap P/N 12130 from Fisher that does the same thing. It is a bit cheaper and is actually a double .4u with a .2u output. This is a clear capsule and comes marked as "Sterile". I use this with one tank of sheath (7 liters). Because I sort cells for patient use I dispose of the filter after the 7 liter usage. jim -----Original Message----- From: Dr S Sreckovic [mailto:Sasa.Sreckovic@bristol.ac.uk] Sent: Monday, September 20, 1999 10:42 AM To: cyto-inbox Subject: PALL Hi dear folks, Does anyone out there in Sortland use PALL system of autoclavable sheath filters on Vantage (or other machine)? What's your opinion of this system comparing with frequent changing of ordinary filters in order to maintain sterility on sorter. Thanks everyone, Sasha ---------------------- Dr Sasha Sreckovic Dept Path & Micro University of Bristol University Walk Bristol, BS8 1TD, UK Sasa.Sreckovic@bristol.ac.uk 0117-928-8606
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