On the FL-A 1999 CAP Survey, I utilized SimulSet software and for the first time a high percentage of my values are higher than the survey values (exihibited positive bias). I reanalyzed my original data (from FL-A) using CellQuest and in the main the values all dropped but were both positive and negative around the survey mean. Even though for years flow analysis using SimulSet has never produced values with a tendency toward positive bias, when I received CAP Survey FL-B 1999, I used CellQuest software for my analysis. In the case of FL-B and CellQuest, my values in the main reflect a negative bias. Not that the values are too low just that a high percentage are lower than the survey mean. Has anyone had similar experiences with SimulSet and CellQuest software? Please provide me with any insight you have or experiences you have had with SimulSet and CellQuest software. The computer involved in the 1999 CAPs was a FACStation--in all other past clinical flow analysis and CAPs, I have used a Hewlett-Packard Consort 32. Linda T. Archer, Ph.D., Director Flow Cytometry, VAMC, OKC
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