Hello all. Our lab here received new lots of CD19 PE (lot # 11841) and CD3 FITC (lot #10799) from BD recently Both of these antibodies appear to be staining the negative cells, giving us incorrect statistics if we don't move the quadrant markers. A parallel test of these two antibodies to previous lots was not done. I was wondering if anyone else has received these lots and are experiencing the same problems of the negative cells becoming dimly positive for CD19 or CD3? I think a titer needs to be done but our lab director feels that isn't necessary. I've double checked instrument settings and re-stained many samples. Can anyone offer me some advice or insight? I don't know what is acceptable to do to make the negative cells move back down into the corner. We are seeing this happen on both stains on peripheral blood and bone marrow. I can provide our staining protocol or any other information to those who are interested. Thank you in advance, Lora Barsky
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