Re: Best filter for P.I.

From: Art Roberts (robertar@UMDNJ.EDU)
Date: Wed Sep 22 1999 - 14:08:14 EST

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I dug out some old records of a former colleague who had optimized a
filter configuration for 2-color analysis of propidium iodide (PI) and
FITC on a Coulter Profile (which allows a maximum of 2 colors). Instead of
the usual FITC/PE setup, he used:

split:  560SPdi
FL1:    530SP
FL2:    610LP

Given the better spectral separation achieved by this combination,
probably much less color compensation would be required while giving
equivalent or better fluorescence intensities.  Are there any thoughts on
this?

AR
__________________________________
Arthur Roberts
Dept. of Medicine
Robert Wood Johnson Medical School

email:  robertar@umdnj.edu
phone:  732-235-7790
Fax:    732-235-7792

Next message: Barsky, Lora: "CD19 PE and CD3 FITC behaving strangely?"
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In reply to: Raymond B. Hester: "Best filter for P.I."
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