Matthias, regarding small scale hybridoma bioreactors, we have experience using the CL1000 from Integra. The system is easy to handle and use, and gives a good amount of antibody in short time. The CL1000 has a cell compartment of about 10-15 mL, holding up to 10^9 cells, supported by 1L of medium on the outside. We harvest and change the medium twice a week. A 4 week run gives us about 50-100 mg of mAbs. Integra also have two smaller systems in this "CELLine" series, I believe it is with 350 and 30mL outer volume (5 and 0.6 mL inner vol., if I remember correct) Link to Integra's CELLine pages: http://www.integra-biosciences.com/e-fcelline.html Hope this is helpful Hanne Mette Dyrlie Kristensen ----------- Diatec as Gaustadalléen 21 0349 OSLO NORWAY Tel: (+47) 22 95 83 87 Fax: (+47) 22 95 86 49 hanne.mette.dyrlie@diatec.com http://www.diatec.com -----Original Message----- From: Matthias Haury [mailto:mhaury@igc.gulbenkian.pt] Sent: Wednesday, September 08, 1999 8:23 PM To: cyto-inbox Subject: Hybridoma mAb production mini-bio-reactors Hello everybody, I would like to buy one of this hybridoma mini-bio-reactors that you can use to obtain on a small scale high mAb concentrations for custom mAb production and purification. I have heard that some of this machines are easier to use than others and have less risks of contamination... therefore I would like to know if some of you have experience with this and what model of those culture chambers you are using. Just to make myself clear, I don't want a real bio-reactor but one of this devices that allow you to grow high density of hybridomas and recovering the supernatents with high antibody concentrations, typically to produce a couple of mg of an custom antibody in concentrations of about 10 mg/ml... I'd be very happy to get some suggestions on which are the better of those devices. Thanks very much in advance. If others are interested I can post the summary to the list (just send me a mail directly). cheers, Matthias _____________________________________ Dr. Matthias Haury Instituto Gulbenkian de Ciencia Unidade de Imagiologia Celular Rua Da Quinta Grande N° 6 - Apt.14 P-2781 Oeiras Codex Portugal Email: mhaury@igc.gulbenkian.pt Tel: + 351 (01) 440 79 85 Office Tel: + 351 (01) 440 79 10 Secretary Fax: + 351 (01) 440 79 70 _____________________________________
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