Matthias and anyone else who might be interested, I run a core facility which produces custom monoclonal antibodies for our investigators and over the last year I have almost eliminated the use of mouse ascites for the production of antibodies. I have used many of the in vitro systems available. I have found the CellLine devices (http://www.integra-biosciences.com/e-fcelline.html) manufactured by Integra Biosystems far superior to the other devices when it comes to cost and the ease of use. I have had especially good results with the larger devices (CL1000 and CL350) as compared to the 6 well device (CL6well). It is harder to keep the 6 well device contamination free as long as the other devices. The cost per device is about $100-150USD. As far as concentration of antibody produced, Integra claims that they get 1-2mg/ml, but with my hybridomas, I have been getting 0.5-1.0mg/ml. Feel free to give me a call for more information about my experience with these devices. They aren't quite as efficient as the mouse ascites method, but in a climate where IACUC committee's are more reluctant to approve the generation of mouse ascites, for me has been a viable alternative. Regards, Bradley ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Bradley Bone Immunology and Research Coordinator Mayo Clinic Scottsdale Phone: 480-301-7131 Pager: 480-301-5023 Email: bone.bradley@mayo.edu -----Original Message----- From: Matthias Haury [mailto:mhaury@igc.gulbenkian.pt] Sent: Wednesday, September 08, 1999 11:23 AM To: cyto-inbox Subject: Hybridoma mAb production mini-bio-reactors Hello everybody, I would like to buy one of this hybridoma mini-bio-reactors that you can use to obtain on a small scale high mAb concentrations for custom mAb production and purification. I have heard that some of this machines are easier to use than others and have less risks of contamination... therefore I would like to know if some of you have experience with this and what model of those culture chambers you are using. Just to make myself clear, I don't want a real bio-reactor but one of this devices that allow you to grow high density of hybridomas and recovering the supernatents with high antibody concentrations, typically to produce a couple of mg of an custom antibody in concentrations of about 10 mg/ml... I'd be very happy to get some suggestions on which are the better of those devices. Thanks very much in advance. If others are interested I can post the summary to the list (just send me a mail directly). cheers, Matthias _____________________________________ Dr. Matthias Haury Instituto Gulbenkian de Ciencia Unidade de Imagiologia Celular Rua Da Quinta Grande N° 6 - Apt.14 P-2781 Oeiras Codex Portugal Email: mhaury@igc.gulbenkian.pt Tel: + 351 (01) 440 79 85 Office Tel: + 351 (01) 440 79 10 Secretary Fax: + 351 (01) 440 79 70 _____________________________________
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:58 EST