Re: CLL and Proliferation

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Wolfgang Beisker writes:
>
>we want to look for proliferating B-cells in blood from patients with a CLL. 
>The proliferating fraction we see in the ficoll prepared blood is about 0.1 
>to 0.3%. Are there any experiences with sorting this fraction out or enrich 
>it? Because for sorting this results in only about 1 to 3 cells per second in 
>a standard FACS, which is rather poor.
>

I would try centrifugal elutriation, which is pretty good at enriching for
S/G2/M phase cells.

-Howard

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