We also have tried this antibody in parallel with Annexin V without success but haven't done a time course, etc to determine if there are biological reasons this isn't working for us. I would be interested in others experience with this antibody and any tricks people may have used to get it to work better. Maryalice >Dear all > >We have been using a anti-Caspase-3 rabbit polyclonal ab conjugated >with PE (this is supposed to bind to the active form only) by flow. >We've looked for caspase-3 activation in camptothecin or etoposide >treated Jurkatt cells (cytospins confirm apoptosis in these >cells) after fixation and perm, without success. This ab is from >Pharmingen. > >Does anyone have experience with looking for caspase-3 activation by >flow ? Are there other antibodies available ? > >Any comments would be much appreciated. > >Fran Gibson >Dept Haematology >St. George's Hospital Medical School >London Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH
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