caspase-3

From: frances gibson (fgibson@sghms.ac.uk)
Date: Fri Aug 20 1999 - 10:21:36 EST

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Dear all

We have been using a anti-Caspase-3 rabbit polyclonal ab conjugated 
with PE (this is supposed to bind to the active form only) by flow. 
We've looked for caspase-3 activation in camptothecin or etoposide 
treated Jurkatt cells (cytospins confirm apoptosis in these 
cells) after fixation and perm, without success. This ab is from 
Pharmingen.

Does anyone have experience with looking for caspase-3 activation by 
flow ? Are there other antibodies available ?

Any comments would be much appreciated.

Fran Gibson
Dept Haematology
St. George's Hospital Medical School
London

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