Hello all, I recently attended a apoptosis/cytokine workshop sponsored by Pharmingen in July. One of the technical application specialists said they (Pharmingen) were gathering data to support that it was possible to run both tests on the same sample, one followed by the other, provided you set up the samples a particular way. Maybe a call to customer service, or technical application support, would help. Of course, this data was not published yet. Good Luck Lora Barsky Children's Hospital Los Angeles > -----Original Message----- > From: Maryalice Stetler-Stevenson [SMTP:stetler@box-s.nih.gov] > Sent: Tuesday, September 07, 1999 6:35 AM > To: Cytometry Mailing List > Subject: Re: caspase-3 > > > We also have tried this antibody in parallel with Annexin V without > success > but haven't done a time course, etc to determine if there are biological > reasons this isn't working for us. I would be interested in others > experience with this antibody and any tricks people may have used to get > it > to work better. > > Maryalice > > >Dear all > > > >We have been using a anti-Caspase-3 rabbit polyclonal ab conjugated > >with PE (this is supposed to bind to the active form only) by flow. > >We've looked for caspase-3 activation in camptothecin or etoposide > >treated Jurkatt cells (cytospins confirm apoptosis in these > >cells) after fixation and perm, without success. This ab is from > >Pharmingen. > > > >Does anyone have experience with looking for caspase-3 activation by > >flow ? Are there other antibodies available ? > > > >Any comments would be much appreciated. > > > >Fran Gibson > >Dept Haematology > >St. George's Hospital Medical School > >London > > Maryalice Stetler-Stevenson > Director Flow Cytometry Unit > Laboratory of Pathology, NCI, NIH
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