Hi again, As I posted just last week, we did this successfully in the early 90's using electroporation, getting sorted labelled live cells back; method published in Cytometry and J Imm Meth. (1990 or 91). Also in Howard's second version. Deb Berglund Montana State University On Thu, 26 Aug 1999, Shirin Munir wrote: > > > I was wondering to find some cell permeabilizing protocol. My aim is to > stain an intracellular protein by indirect fluorescence antibody staining > but at the same time I don't want to kill the cells during fixing process. > Do you know of any cell permeabilizing method to stain intracellular > protein which will stain the cell but cell will stay viable. > > Thank you very much for your help > > > Shirin Munir > > Dept. of Veterinary PathoBiology > University of Minnesota > 1971 Commonwealth Ave, St. Paul MN 55108 > > tel:612-624-8709 > fax:612-625-5203 > > > > >
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