intracellular staining of viable cells

From: Shirin Munir (munir001@tc.umn.edu)
Date: Thu Aug 26 1999 - 10:35:51 EST 

I was wondering to find some cell permeabilizing protocol.  My aim is to 
stain an intracellular protein by indirect fluorescence antibody staining 
but at the same time I don't want to kill the cells during fixing process.
Do you know of any cell permeabilizing method to stain intracellular 
protein which will stain the cell but cell will stay viable.  

Thank you very much for your help


Shirin Munir

Dept. of Veterinary PathoBiology
University of Minnesota
1971 Commonwealth Ave, St. Paul MN 55108

tel:612-624-8709
fax:612-625-5203

This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:54 EST