Re Lora Barsky's advice:
> I kick myself for not checking the filter sets earlier. I discussed these
> issues with my field engineers and we agreed that a 575BP26 should work for
> PE, but really, it doesn't. I was loosing a lot of sensitivity!!!
>
> Hoping others may learn from my experiences, I'm giving the following
> advice: be sure to run PE with 585BP42 filter set in FL2 on the
> FACSVantage, when using an Enterprise Argon/UV laser.
We use a 575BP26 filter for FL2 on our FACStar because of the presence
of a dye laser (as described by Marty Bigos) but recently went testing
our PE sensitivity. Swapping in a 585BP42 filter did indeed give us
more signal but also proportionately more background so that the
signal-to-background was _not improved_. I guess we could have
expected this since the narrower filter is on the PE emission peak
where the signal-to-background is highest. The wider filter gets more
of the "skirt" region where the signal-to-background is lower.
Frank.
\ / < The Cytometry Laboratory
Frank Battye, \__/ <<<<< The Walter & Eliza Hall Institute
Dora Kaminaris,----------!!<<<<<<<< Victoria 3050, AUSTRALIA
Viki Lapatis and /!!\ <<<<< ph: 61_3_9345 2540 fax: 61_3_9347 0852
Jennie Parker o !! \ < in:: "facs_copy@wehi.edu.au"
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