Detection of giardia and cryptosporidium

From: Lemoenic.Steve@EPAMAIL.EPA.GOV
Date: Thu Aug 26 1999 - 11:02:04 EST


Lezlie,

There are a few groups working in this area including ourselves. For a
comprehensive list of references try this site at Macquarie University in
Sydney.

http://www.bio.mq.edu.au/flowgird/

Good luck,
Steve


Dear all,

           I am a postgraduate research student who is working on a project
involved in detecting giadia and cryptosporidium using flow cytometric
methods. I would like to inquire if anyone has had experiences in using
fluorescein-conjugated monoclonal antibodies for such purposes and if anyone
knows of some method of using such antibodies with the flow cytometer. For
example what's the procedure of sample preparation and incubation time, etc.


Kind Regards
Lezlie Neo Sim Yee
National University of Singapore
Civil Engineering Department


***************************************************
Steven Le Moenic
Office of Ground Water and Drinking Water,
U.S.EPA
Cincinnati, OH, 45268



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