PBS without calcium and magnesium cannot support the growth of microorganisms and does not need supplementation with antibiotics. If unopened, to avoid spore contamination, it can be stored indefinitely at room temp. Saverio Alberti Head, Lab. of Experimental Oncology Department of Cell Biology and Oncology Consorzio Mario Negri Sud 66030 Santa Maria Imbaro (Chieti), Italy Phone: (39) 872-570.293 FAX: (39) 872-570.412 E-mail: alberti@cmns.mnegri.it On Mon, 23 Aug 1999, Elaine Kunze wrote: > > To follow up a bit on the sheath fluid questionaire: > > Do you routinely put antibiotics in homemade PBS? If so, what do you use? > How long is the PBS stored or do you make it fresh? > **************************************************************************** > Elaine Kunze > Flow Cytometry.....Image Analysis... > The Biotechnology Institute for Research and Education > Life Sciences Consortium > 8B Althouse Laboratory (814-863-2762) > Penn State University > >
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