If you want live cells back, we did this sort of thing in 1990 or 91 (methods published in either Cytometry or J Imm Meth) by using electoporation. Get back to me if you need specifics. Deb On Mon, 23 Aug 1999, Liz Seftor wrote: > > Justin Fishbaugh (justin-fishbaugh@uiowa.edu) here at the University of > Iowa Flow Cytometry core suggested I email you directly to ask if you know > of any possible protocols for sorting cells by labeling a cytoplasmic > protein (i.e. intermediate filament proteins like keratins or vimentin)? I > would appreciate any suggestions you can make. We need to enrich our cell > population for only those cells that are expressing a particular > intermediate filament for upcoming molecular work. > > Thanks - Liz Seftor >
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