On Wed, 25 Aug 1999 11:54:37 +0200 (MET DST) Saverio Alberti <alberti@cmns.mnegri.it> wrote: > PBS without calcium and magnesium cannot support the growth of > microorganisms and does not need supplementation with antibiotics. If > unopened, to avoid spore contamination, it can be stored indefinitely at > room temp. Hi, Saverio, Sorry, but I can't let that pass without comment. As an environmental microbiologist, who uses flow to sort out individual bacteria with the sole aim of growing them, there are many bacteria and some fungi that will grow in PBS or Dulbecco PBS, or even deionised and x1 distilled water. They grow to very low densities, and the solutions remain clear to the eye, but I filter my sheath fluid through a 0.1 um filter, aliquot it, autoclave it and would never return to an opened flask. Of course, these cells do not normally affect the work of most of the people who subscribe to the list as a) they are unlikely to grow in the incubation conditions you use and b) you all work with golf balls and footballs and rarely take your instruments into the bit you call noise. I also do other odd things, but haven't (yet) been arrested for them... Jonathan
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