Hi folks, To those with expertise and experience using PI to look at sub 2N DNA content as a measurement of apoptosis, I have a fairly basic question that I hope you can answer. Does this assay definitively distinguish between apoptosis and necrosis? I guess these days suspicion arises in the field of cell death because almost everything is decribed as apoptosis. I just don't know if the PI hypodiploid assay gives one the same DNA fragmentation readout as say TUNEL or a laddering assay. If the PI assay is specific for apoptosis, what would a necrosis profile look like in comparison? Thanks in advance to any who can help me with an answer. This group is truly fantastic! best regards, James Liou
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