Hi All, Forgive me if this has been discussed before. We do 3-color staining (CD3/4/8) on coulter profile 2 to monitor HIV for clinical purposes, using coulter reagents (3 mca in 1 vial). We derive absolute values from simultaneous FBC and diff which adds to cost. Has anyone succeeded in getting absolute CD4 values directly from flow cytometer, while using 3 color reagents? I would value any feedback. Thanks Stanley Ress Head: Clinical Immunology laboratory Department of Medicine H47 Old Main Building-room 26 Groote Schuur Hospital and UCT Observatory 7925 Cape Town South Africa TEL:INTERN. + 2721-4066201 or 4066197 FAX: " + 2721-4486815 e-mail: SRESS@uctgsh1.uct.ac.za
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