The best way to track the situation is to covalently label the cells and then do your antibody staining. There was an excellent paper given about the theorie of clonal expansion on the Sam Latt conference (you all missed out on an excellent conference if you were not there) by Phillip Hodgkin from Sydney. He combined CFDA-succinimidylester labelling with antibody staining and demonstrated the time dependancy of clonal expansion in a mathematical model. Pitty there isn't a printed summary, but I look forward to the paper. Gerhard -----Original Message----- From: Tony Schountz [SMTP:tschount@mesastate.edu] Sent: Friday, August 13, 1999 5:53 PM To: Cytometry Mailing List Subject: Opinions on proliferation by flow I'd like opinions on the use of flow for the detection of T cell proliferation. I know that bromodeoxyuridine is an established method, but right now out of my reach ($$). My thought is to use anti-CD3, -CD4 and/or -CD8 on bulk lymph node cultures stimulated with or without antigen to get changes in total numbers of cells. Thanks, Tony -- Tony Schountz, Ph.D. Department of Biological Sciences Mesa State College mailto:tschount@mesastate.edu
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