Opinions on proliferation by flow

From: Tony Schountz (tschount@mesastate.edu)
Date: Fri Aug 13 1999 - 11:52:44 EST 

I'd like opinions on the use of flow for the detection of T cell
proliferation. I know that bromodeoxyuridine is an established method,
but right now out of my reach ($$). My thought is to use anti-CD3, -CD4
and/or -CD8 on bulk lymph node cultures stimulated with or without
antigen to get changes in total numbers of cells.

Thanks,

Tony

--
Tony Schountz, Ph.D.
Department of Biological Sciences
Mesa State College
mailto:tschount@mesastate.edu

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