Hello Everyone, I am trying to find a protocol for fixing and permeabilizing mouse erythrocytes to allow intracellular staining for fetal hemoglobin levels with a HbF-FITC antibody. I can only assume that C57Bl/6 RBCs are significantly more susceptible to hemolysis than human RBCs. People have treated human RBCs for hours at room temp in 4% formaldehyde before permeabilization with Triton. Mouse RBCs lyse after short duration exposure to 4% formaldehyde at 4 degrees and a single pelleting at low speed. Any ideas on how to handle such a fickle beast would be greatly appreciated?!! Dana Dana Levasseur Dept. of Biochemistry and Medical Genetics Bevill Biomedical Research Building, Rm 263 University of Alabama at Birmingham Birmingham, Alabama 35205 e-mail: dnl@uab.edu
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