Dear Mark "Cryopreservation and Long-Term Liquid Nitrogen Storage of PBMC for Flow Cytometry Analysis:Effects on Cell Subset Proportions and Fluorescence Intensity" Journal of Clinical Laboratory Analysis 5:255-261(1991) Let me know if you want me to mail you a copy--Kristin At 02:36 PM 7/23/99 -0400, you wrote: > >Hello group, >Concerning the effect of freezing and thawing on immunophenotype, say >for leukocyte subsets . . . all I have are personally communicated >impressions (perhaps higher background, loss of cells, antigen >expression changes). Are there published comparisons of differences, if >any? I've searched without success, yet . . . still looking. Any >references making comparisons of frozen to fresh, perhaps looking to >optimize, would be greatly appreciated. >MAK. > >-- >Mark A. KuKuruga, Managing Director >University of Michigan Core Flow Cytometry >kukuru@umich.edu > > >
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