anti-TdT staining

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Dear Flowers,

     I have a few questions about anti-TdT that hopefully you can answer.

1.   What are the recommended cell lines to use as a postive control and
what does the staining profile look like.  ( I tried an EBV transformed
BLCL RT-PCR+ for TdT but am not convinced by my results.)

2.  What are the preferred techniques and reagents that people are using.
(Anything other than Triton, Tween, BD FACS LYSE, Saponin, NP40?)

3.  What does the staining profile of normal human thymus, LN and bone
marrow look like?  Are there clearly positive populations or just a small
MFI shift.

4.  Mice people - What monoclonal do you use and what does mouse thymus
look like?

     Thanks for any info.

Sincerely,

Craig Irwin
ADARC

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