I want to thank everybody again for their replies on the sub G0 peak on the Hoechst/Pyronin stain. Some of you also asked for a protocol and setup for this procedure. My e-mail has gone awry in the meantime and therefore I do not have all the addresses of the people that specifically requested the protocol and with an apology to the rest of the subscribers, I will include the protocol below in a Word97 attachment. The laser is an Innova set to UV with the appropriate couplers and a 424 nm filter on FL4. We routinely do it with a FITC stain and that works well. Hope this helps. > <<HST.DOC>>
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