Thomas Delohery wrote: > Mark KuKuruga said: > >I think that the flow cytometry community in general has overemphasized the > difficulties arising from the use of AO. Much >(if not all) of this is > unwarranted. In my opinion, AO is extremely useful for detecting perturbations > to the cell cycle, perhaps >second to BrdU. It's perhaps the best way to detect > the transition from G0 to G1. Its advantage is that it is probably much > >easier to develop. > > Given our current knowledge of the molecular mechanisms involved in cell cycle > regulation (and cell death) it's highly doubtful that an AO fluorescence assay > purported to measure G0 to G1 transitions would be accepted without additional, > independent assays and data. > td Thomas,What are you taking about? What fo you mean by "measure?" Do you dispute the relative change that occurs in a quiescent population upon stimulation to proliferate, as detected by AO? Have you looked at the supporting literature that validates this phenomenon? Have you done these experiments, and observed these changes first hand? I think it's time you showed us some "independent assays and data" to support your opinion that AO does not do what that extensive body of literature suggests. MAK. -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry kukuru@umich.edu
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