Mark KuKuruga said: >I think that the flow cytometry community in general has overemphasized the >difficulties arising from the use of AO. Much (if not all) of this is >unwarranted. In my opinion, AO is extremely useful for detecting >perturbations >to the cell cycle, perhaps second to BrdU. It's perhaps the best way to >detect >the transition from G0 to G1. Its advantage is that it is probably much >easier >to develop. Given our current knowledge of the molecular mechanisms involved in cell cycle regulation (and cell death) it's highly doubtful that an AO fluorescence assay purported to measure G0 to G1 transitions would be accepted without additional, independent assays and data. td -- ============================================================================== Thomas M. Delohery |Internet: t-delohery@ski.mskcc.org Supervisor, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (917) 432-2333 1275 York Ave. Box 98 | New York, NY 10021 | ==============================================================================
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