I have yet to meet someone who never asks dumb questions. I ask my share and I am proud that I am not afraid to ask a dumb question. At least I get the needed information when I need it. I don't, however, feel that the original CLL question was dumb. CLL is difficult to detect Kappa/lambda staining. We always use at least 2 different light chain reagents because sometimes one will give you an answer and the other won't. Of course we wash the bood but we do a whole blood lysis and we don't incubate the cells (we studied this and didn't find it necessary- Cytometry (Communications in Clinical Cytometry)26:243-252,1996). However sometimes, no matter what you do, the light chain staining is so very dim that it is really hard to detect. I think the discussion on this point was good and I hope all will feel free to join me in asking dumb questions. We will all learn in the process. I like to think that Dirk Van Bockstaele was just kidding and being cute in his reply "Not long in the business I guess? " and not intending to insult. Let's cut him a break too. Maryalice I'm not offended by all the dumb blonde jokes because I know I'm not dumb... and I also know that I'm not blonde. -Dolly Parton- Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH
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