Hi Andrew, why not use the thiazole orange analogue, DEQTC (diethyl quinolyl thiocyanine iodide): to my knowledge it is much less expensive and gives the same results. We have published on that subject : Cytometry 10: 214-216, 1989. Annals Hematology 66: 163, 1993. It is available as product 36811, Fluka catalogue. Best Regards, Dirk Prof. Dirk Van Bockstaele, PhD Laboratory of Hematology Antwerp University Hospital Wilrijkstraat 10 B-2650 Edegem Belgium tel. 32 3 821 3900, fax 32 3 825 1148, e-mail dirk.van.bockstaele@uza.uia.ac.be > ---------- > Van: Andrew Macintyre[SMTP:A.R.Macintyre@liverpool.ac.uk] > Verzonden: maandag 19 juli 1999 23:24 > Aan: Cytometry Mailing List > Onderwerp: Retics and PI > > > Dear Colleagues, > Two questions that I need a bit of help with. Firstly, measuring > reticulocytes, I have used an thiazol orange method on human RBC's > sometime ago. Is this still the best method of choice?. Also is it > possible to class the retics into groups i.e. of differing staining > intensity. Any comments from people performing retics routinely most > welcome. Secondly, a student would like to perform some PI staining > (looking at ploidy) in some lymphoma paraffin blocks. Removing the > paraffin is not a problem but the digestion to a single cell suspension. > I would appreciate any fool proof ! methods for digesting the tissue. > Many thanks, > > Andy Macintyre. > Department of Veterinary Pathology. > > >
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